欧美另类视频-欧美另类性-欧美另类杂交a-欧美另类重口-欧美另类专区-欧美乱妇

咨詢(xún)熱線(xiàn)

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>IMR-90人胚肺成纖維細(xì)胞

人胚肺成纖維細(xì)胞

簡(jiǎn)要描述:CCL-186 IMR-90 人胚肺成纖維細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):IMR-90
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-06-26
  • 訪(fǎng)  問(wèn)  量:3155

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

CCL-186 IMR-90人胚肺成纖維細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

CCL-186 IMR-90人胚肺成纖維細(xì)胞.

ATCC® Number: CCL-186™

Designations: IMR-90

Depositors: WW Nichols

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: fibroblast

CCL-186 IMR-90人胚肺成纖維細(xì)胞.

Source: Organ: lung

Disease: normal

Cell Type: fibroblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications: transfection host (Nucleofection technology from Lonza)

Virus Susceptibility: Human poliovirus 1

Human poliovirus 2

Varicella-Zoster

Herpes simplex virus 1

Herpes simplex virus 2

Human poliovirus 3

Vaccinia virus

Human herpesvirus 5

Vesicular stomatitis virus

Reverse Transcript: negative

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,13

D13S317: 11,13

D16S539: 10,13

D5S818: 12,13

D7S820: 9,12

THO1: 8,9.3

TPOX: 8,9

vWA: 16,19

Cytogenetic Analysis: normal human female; diploid; stable

Isoenzymes: G6PD, B

Age: 16 weeks gestation

Gender: female

Ethnicity: Caucasian

Comments: The human diploid fibroblast strain IMR-90 was derived by W.W. Nichols and associates from the lungs of a 16-week female fetus. [22381]

The division potential, viral susceptibilities and other properties have been thoroughly studied such that the line may be considered as an alternate for WI-38 and other standard human lung cell strains.

The cells have been reported to be capable of attaining 58 population doublings before the onset of senescence. [22381]

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:8 is recommended

Medium Renewal: Every 3 to 4 days

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 22381: Nichols WW, et al. Characterization of a new human diploid cell strain, IMR-90. Science 196: 60-63, 1977. PubMed: 841339

32932: Dolganov GM, et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair. Mol. Cell. Biol. 16: 4832-4841, 1996. PubMed: 8756642

33041: Ostlund RE Jr., et al. A sterospecific myo-inositol/D-chiro-inositol transporter in HepG2 liver cells. J. Biol. Chem. 271: 10073-10078, 1996. PubMed: 8626564

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products


IMR-90人胚肺成纖維細(xì)胞更多信息,請(qǐng)致電咨詢(xún)。


















 

產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話(huà):

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:[email protected]

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線(xiàn)

400-821-8510

(周一至周日9:00- 19:00)

在線(xiàn)咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 国产喷水在线观看 | 欧美精品一区二区免费开放 | 精品久久人人做人人爽综合 | 国产成人精品亚洲午夜麻豆完整版 | 东京热无码aⅴ一区二区 | 亚洲欧美日韩尤物aⅴ一区 亚洲欧美日韩在线不卡中文 | 亚洲av无码成人精品区在线观看 | 国产欧美一区二区精品仙草咪 | 欧美亚洲偷图色综合 | 亚洲精品视频在线播放 | 国产精品成久久久久三级 | 爽欲亲伦小说 | 国产一区二区三区成人久久片 | 毛片网站网址 | 欧洲精品亚洲精品国产精品 | 国产成人高清视频在线观看免费97 | 91麻豆精品国产专区在线观看 | 欧美日本免费一区二区三区 | 国产成人一区久久 | 久久久久久久久久久9精品视频 | 18禁动漫一区二区三区免费下 | 国产欧美日韩精品一区二区三区 | 成人a级毛片免费播放 | av黄色网址在线观看 | 欧美孕妇变态重口另类 | 国产亚洲欧美日韩三级 | 人妻av中文字幕无码专 | 青草青华人在线观看视频 | 伊人一区二区三区四区 | 日韩x做受欧美 | 成人黄色网站视频色 | 久久久久久久久久中文字幕 | 日本视频免费观看的网站 | 国产手机精品一区二区 | 久久国产香蕉女人 | 精品一区二区日本高清 | 日本高清激情乱一区二区三区 | 成熟少妇AV片在线观看 | 亚洲欧美日韩国产另类一区二区三区 | 波多野结衣在线观看 | 日本玖玖视频 |